2023 World Conference on Lung Cancer (Posters)-EP02.02. Radiation-induced SERPINE2 Expression Inhibit Abscopal Effect in Non-small Cell Lung Cancer via Exosomes

EP02.02. Radiation-induced SERPINE2 Expression Inhibit Abscopal Effect in Non-small Cell Lung Cancer via Exosomes - PDF(Abstract)

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This study investigates the role of SERPINE2 expression and exosomes in inhibiting the abscopal effect in non-small cell lung cancer (NSCLC) after radiation therapy. The abscopal effect refers to the systemic anti-tumor effect of radiotherapy. The study establishes bilateral tumor xenograft models and detached co-culture systems to explore the occurrence of the abscopal effect. However, no significant abscopal effect is observed in both in vivo and in vitro studies.<br /><br />RNA sequencing (RNAseq) is performed on irradiated and non-irradiated NSCLC cells, and it is found that SERPINE2 expression is significantly increased after radiation. Exosomes derived from the irradiated NSCLC cells also show high SERPINE2 expression. To further investigate its role, SERPINE2 overexpression and knockout cells are created. The overexpression of SERPINE2 promotes the proliferation of lung cancer cells, and exosomes from these cells also enhance the proliferation of co-cultured lung cancer cells.<br /><br />Transcriptome analysis reveals that the differential genes are enriched in the ERK/MAPK signaling pathway. Western blot shows elevated levels of p-ERK and p-AKT in lung cancer cells co-cultured with high SERPINE2 exosomes. <br /><br />Overall, this study uncovers the mechanisms by which radiation-induced SERPINE2 expression inhibits the abscopal effect in NSCLC. It demonstrates that exosomal SERPINE2 regulates the ERK/MAPK signaling pathway, promoting the proliferation of abscopal cells. Understanding these mechanisms is crucial for better utilizing the systemic anti-tumor effect of radiotherapy and may provide evidence for improving the low occurrence of the abscopal effect in clinical settings.

Asset Subtitle

Lucheng Zhu

Meta Tag

Speaker Lucheng Zhu

Topic Tumor Biology: Preclinical Biology - Omics Approaches

Keywords

SERPINE2 expression

exosomes

abscopal effect

non-small cell lung cancer

NSCLC

radiation therapy

bilateral tumor xenograft models

RNA sequencing

proliferation of lung cancer cells

ERK/MAPK signaling pathway