2023 World Conference on Lung Cancer (Posters)-EP02.02. Transcriptomic Response to Tumor Treating Fields (TTFields) Across Tumor Types

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In this study, researchers investigated the transcriptomic response to Tumor Treating Fields (TTFields) across different tumor types. TTFields are electric fields that disrupt the function of polarized molecules in cancer cells, leading to anti-mitotic effects. Previous studies have also shown that TTFields can affect DNA repair, replication stress, autophagy, and immunogenic cell death. The goal of this study was to identify common responses to TTFields across different cancers.<br /><br />The researchers used samples from various tumor types, including non-small cell lung carcinoma, glioblastoma, ovarian cancer, pancreatic cancer, gastric cancer, malignant pleural mesothelioma, and hepatocellular carcinoma. They analyzed the differentially expressed genes between treated and control samples and calculated the correlation between the datasets. They identified significantly overlapping pathways using the ActivePathways package and conducted enrichment analysis according to the Gene Set Enrichment Analysis (GSEA) of Molecular Signatures Database (MSigDB).<br /><br />The results showed a positive correlation in the expression of genes and pathways in response to TTFields across different tumor types. Pathway-based analysis was found to have a stronger correlation compared to gene-based analysis. The commonly affected pathways included cell cycle, DNA repair and replication, and metabolism of RNA and proteins.<br /><br />Furthermore, the response to TTFields led to the upregulation of genes associated with tumor suppression, cellular transport, and metabolism. It also downregulated cell cycle-related pathways, indicating cell cycle arrest, and upregulated pathways associated with the immune response.<br /><br />In conclusion, this study revealed common pathways involved in the response to TTFields across different tumor types. These pathways were related to cell cycle arrest, DNA repair inhibition, upregulation of the immune response, and RNA and protein metabolism. The findings provide new insights into the potential mechanisms of action of TTFields and suggest areas for further investigation.

Asset Subtitle

Moshe Giladi

Meta Tag

Speaker Moshe Giladi

Topic Tumor Biology: Preclinical Biology - Omics Approaches

Keywords

TTFields

transcriptomic response

common responses

DNA repair

cell cycle

immune response

RNA and protein metabolism

tumor suppression

cellular transport

enrichment analysis