2023 World Conference on Lung Cancer (Posters)-EP02.04. Xenotransplanted Lung Tumors Display Variable Pleomorphic Patterns in Immunosuppressed BALB/c Mice

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Researchers at the University of the Philippines Manila and the University of California Davis have developed a xenotransplantation model for studying lung cancer in immunosuppressed BALB/c mice. The model involved injecting A549 lung cancer cells and patient-derived lung adenocarcinoma cells into the mice. The mice were then observed for tumor growth and subjected to histopathological and immunohistochemical analysis.<br /><br />The researchers found that the mice exhibited general lethargy, with the A549-injected mice exhibiting a moribund phenotype after 2 weeks. The patient-derived cells lasted for more than 30 days prior to dissection. Immunohistochemistry-based confirmation with TTF1 and TP53, however, did not show positive detection. The researchers concluded that further investigation is needed to determine whether the modification of tumor morphology and absence of TTF1 and TP53 expression are due to tumor cell-mouse environment interaction or early-stage tumor development.<br /><br />In terms of histopathology, the researchers observed extensive tumor-like cells in various organs, with more tumor-like growth seen in the A549-injected mice compared to the patient-derived cells. In situ fluorescence microscopy using labeled antibodies confirmed the presence of pleomorphic cells in the lungs and other tissues, suggesting that most of the injected cells migrated to the lungs of the animals.<br /><br />The researchers believe that this xenotransplantation model can simulate tumor metastasis and provide further insight into the heterogeneity of lung cancer tumors. The model is also more affordable and accessible than using athymic mice. The research was funded by the Philippine Commission on Higher Education and received support from various institutions and laboratories.<br /><br />Overall, the study demonstrates the potential of this xenotransplantation model for studying lung cancer and guiding appropriate therapeutic modalities. Further research is needed to better understand the behavior of the tumors in this model and to explore its potential applications in drug testing and personalized medicine.

Asset Subtitle

Maria Isabel Idolor

Meta Tag

Speaker Maria Isabel Idolor

Topic Tumor Biology: Preclinical Biology - Tumor Immunity

Keywords

xenotransplantation

lung cancer

immunosuppressed mice

A549 cells

patient-derived cells

tumor growth

histopathology

immunohistochemistry

tumor metastasis

personalized medicine