2023 World Conference on Lung Cancer (Posters)-EP03.03. Comprehensive Analysis of Primary Immunotherapy-resistant Genes with Regard Immune Infiltration and Prognosis in Lung Adenocarcinoma

EP03.03. Comprehensive Analysis of Primary Immunotherapy-resistant Genes with Regard Immune Infiltration and Prognosis in Lung Adenocarcinoma - PDF(Abstract)

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This study focused on the comprehensive analysis of primary immunotherapy-resistant genes in lung adenocarcinoma (LUAD) patients. The researchers characterized the expression of immunotherapy-resistant genes in LUAD samples using publicly available data. They then used consensus clustering analysis to determine immunotherapy-resistant subtypes in LUAD. The researchers also used CIBERSORT to detect the relative proportion of immune cells infiltrating the tumors and used the Tumor Immune Dysfunction and Exclusion (TIDE) score to predict tumor response to immune checkpoint inhibitors.<br /><br />Based on the expression levels of 12 immunotherapy-resistant-associated differential genes, the researchers divided 516 LUAD patients into two subtypes using consistent clustering analysis. They found that Cluster 1 showed lower tumor stage, worse prognosis, and higher expression of the immune checkpoint molecule LAG3 compared to Cluster 2. Cluster 1 also had higher levels of infiltrating immune cells, including Macrophage M1, Macrophage M0, and T regulatory cells. The immune response rate of Cluster 1 was significantly lower than Cluster 2 according to the TIDE algorithm. <br /><br />The researchers also identified a key resistant gene, NT5E, which was highly expressed in LUAD and significantly associated with prognosis. NT5E expression was positively correlated with the expression of CD274 (a gene involved in immune response) and negatively correlated with tumor mutational burden. Additionally, a pan-cancer analysis showed that NT5E was closely related to unfavorable prognosis in various cancer types.<br /><br />In conclusion, this study identified immunotherapy-resistant genes in LUAD and determined specific subtypes associated with resistance. The researchers also identified NT5E as a potential prognostic biomarker and a potential target for cancer immunotherapy.

Asset Subtitle

Jianxiong Deng

Meta Tag

Speaker Jianxiong Deng

Topic Tumor Biology: Translational Biology - IO

Keywords

immunotherapy-resistant genes

lung adenocarcinoma

LUAD

consensus clustering analysis

Tumor Immune Dysfunction and Exclusion

immune checkpoint inhibitors

infiltrating immune cells

resistant gene

NT5E

prognostic biomarker