2023 World Conference on Lung Cancer (Posters)-EP03.03. Immunological Profiling of PD-1-Treated Murine Model Reveals Meyloid-Enriched Phenotypes

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The study presented on the research poster explores the effects of PD-1 antibody therapy on the immune environment in murine models with or without tumor xenografts. PD-1 antibody is a first-line treatment option for lung cancer that activates the tumoricidal role of T cells by inhibiting the immune checkpoint PD-1 on the surface of T lymphocytes. However, the effects of PD-1 antibody therapy extend beyond T lymphocytes.<br /><br />The researchers used Lewis lung cancer cells to generate cell-line derived xenografts in mice. The mice were divided into three groups: a control group, a PD-1-treated group, and a PD-1-treated plus CDX group. PD-1 monoclonal antibody was administered to mice for a 2-week treatment course. After the treatment, various tissues and cells were collected for analysis.<br /><br />The researchers performed CyTOF analysis on the collected samples and observed that CD45 immune cells accounted for over 50% of all cell subsets. However, there were no significant variations in the proportion of cell subsets or gene expression between the different groups.<br /><br />Interestingly, the researchers found that myeloid cell subsets, including CD11b, CD24, and CD44 myeloid cells, were significantly enriched in the overall cell populations. This suggests a strong association between myeloid cell subsets and the response to PD-1 therapy.<br /><br />The study concludes that myeloid derived dendritic cells, macrophages, and inhibitory B cells are the most variable cell subsets in the tumor microenvironment. To further understand the effects of PD-1 on the immune system, more research should focus on other immune components that affect anti-tumor immunity. Additionally, creating a reasonable humanized mouse model could help simulate the immune environment of patients and provide more opportunities for translational medicine.<br /><br />Overall, this study provides insights into the immunological profiling of PD-1-treated murine models and highlights the potential role of myeloid cell subsets in the response to PD-1 therapy.

Asset Subtitle

Xiaoshen Zhang

Meta Tag

Speaker Xiaoshen Zhang

Topic Tumor Biology: Translational Biology - IO

Keywords

PD-1 antibody therapy

immune environment

murine models

tumor xenografts

lung cancer

T cells

myeloid cell subsets

cytometry by time-of-flight analysis

tumor microenvironment

anti-tumor immunity