2023 World Conference on Lung Cancer (Posters)-EP03.03. The Involvement of Tumor Endothelial Cells in the Regulation of PD-L1 and Tregs in the Immune Microenvironment of Early-stage Lung Adenocarcinoma

EP03.03. The Involvement of Tumor Endothelial Cells in the Regulation of PD-L1 and Tregs in the Immune Microenvironment of Early-stage Lung Adenocarcinoma - PDF(Slides)

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This study focused on understanding the role of tumor endothelial cells in the immune microenvironment of early-stage lung adenocarcinoma. The researchers aimed to accurately assess the histologic subtypes of patients at the single-cell level and analyze the microenvironmental differences between different subtypes. <br /><br />The study included 11 postoperative lung adenocarcinoma patients, and single-cell RNA sequencing was performed on the surgical lesions. Additionally, digital spatial Profiler (DSP) and 10X Genomics Visium analysis were conducted on formalin fixation and paraffin embedding (FFPE) slides from selected patients. Regions of interest (ROIs) were identified based on histology and compared with the clinicopathological assessments. <br /><br />The results showed that histologic subtypes could be accurately predicted using the single-cell RNA sequencing data, aligning with the clinicopathologic findings. Differences in the microenvironment between histologic subtypes were identified, with FOXP3, a biomarker for Tregs, showing the highest expression in the normal region and decreasing as tumorigenesis progressed. The expression of FOXP3 then gradually increased with continued tumor progression. <br /><br />The study also found a significant positive correlation between the expression of PD-L1 on endothelial cells and FOXP3 expression, suggesting that endothelial cells may play a role in promoting tumorigenesis and progression by inhibiting immune-responsive CD8 T cells through the expression of PD-L1. This allows tumor cells to evade immune surveillance and killing. <br /><br />In summary, this study highlights the involvement of tumor endothelial cells in the regulation of PD-L1 and Tregs in the immune microenvironment of early-stage lung adenocarcinoma. The findings suggest that endothelial cells may contribute to tumor progression by promoting immune evasion, warranting further investigation into potential therapeutic targets in this context.

Asset Subtitle

Dawei Yang

Meta Tag

Speaker Dawei Yang

Topic Tumor Biology: Translational Biology - IO

Keywords

tumor endothelial cells

immune microenvironment

early-stage lung adenocarcinoma

histologic subtypes

single-cell RNA sequencing

microenvironmental differences

digital spatial Profiler

10X Genomics Visium analysis

FOXP3 expression

PD-L1 expression