2023 World Conference on Lung Cancer (Posters)-EP03.04. Detection of Possible Targets in Aggressive Lung Cancer Using Molecular Abundances and Catalytic Action of Serine Hydrolases

EP03.04. Detection of Possible Targets in Aggressive Lung Cancer Using Molecular Abundances and Catalytic Action of Serine Hydrolases - PDF(Slides)

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Researchers conducted a study to identify possible therapeutic targets in advanced lung cancer by analyzing the molecular abundances and catalytic action of serine hydrolases (SHs). They used activity-based proteome profiling (ABPP) combined with mass spectrometry (MS) to quantify catalytically active enzymes. They developed a novel ABPP method that could simultaneously determine the activity and quantity of hundreds of enzymes by depleting enzymes tagged with a class-specific activity probe and measuring the depleted and non-depleted samples using automated sequential window acquisition of all theoretical spectra (SWATH/DIA-MS). The study analyzed 24 lung adenocarcinoma samples and found that aggressive tumors displayed enhanced lipolysis of metabolic SHs, which could be potential therapeutic targets in lung cancer treatment. The researchers also detected an increase in palmitoleic acid levels in aggressive tumors, suggesting lipid desaturation as a potential energy source for cell proliferation. The study highlights the importance of understanding the functional links between active enzymes and the background proteome in aggressive lung cancer and provides potential strategies for future therapeutic interventions.

Asset Subtitle

Sven Hillinger

Meta Tag

Speaker Sven Hillinger

Topic Tumor Biology: Translational Biology - Novel Targets

Keywords

therapeutic targets

advanced lung cancer

serine hydrolases

ABPP

mass spectrometry

catalytically active enzymes

lipolysis

metabolic SHs

palmitoleic acid levels

lipid desaturation