2023 World Conference on Lung Cancer (Posters)-EP06.03. Usefulness of Immunohistochemistry for the Detection of BRAFV600E in Lung Cancer: A Multicenter Retrospective Study in China

EP06.03. Usefulness of Immunohistochemistry for the Detection of BRAFV600E in Lung Cancer: A Multicenter Retrospective Study in China - PDF(Slides)

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A multicenter retrospective study conducted in China evaluated the usefulness of immunohistochemistry (IHC) for detecting the BRAF V600E mutation in patients with lung cancer. The study aimed to assess the feasibility of using IHC as a screening test for this mutation.<br /><br />The study included 493 patients with non-small cell lung cancer (NSCLC) from six different centers. The BRAF V600E mutation status was determined using real-time polymerase chain reaction (RT-PCR) or next-generation sequencing (NGS). IHC testing using VE1 antibodies was also performed on formalin-fixed paraffin-embedded (FFPE) samples.<br /><br />The results showed that IHC had a sensitivity of 97.2%, specificity of 100%, and consistency of 99.2% compared to molecular testing methods. The study also proposed binary interpretation criteria for the IHC testing of BRAF V600E in NSCLC. The IHC method demonstrated higher consistency with RT-PCR compared to NGS, particularly in resection samples.<br /><br />The authors conclude that IHC using VE1 antibodies is a rapid, inexpensive, and widely available assay that can effectively detect the BRAF V600E mutation in Chinese NSCLC patients. They suggest that IHC could be used as a screening test for this mutation in NSCLC, following extensive validation.<br /><br />Overall, this study provides evidence for the usefulness of IHC in detecting the BRAF V600E mutation in NSCLC. The results support the inclusion of IHC as a screening method in the National Comprehensive Cancer Network (NCCN) guidelines for NSCLC.

Asset Subtitle

Pei Yuan

Meta Tag

Speaker Pei Yuan

Topic Pathology & Biomarkers: Genetic Biomarkers

Keywords

multicenter study

immunohistochemistry

BRAF V600E mutation

lung cancer

screening test

non-small cell lung cancer

VE1 antibodies

molecular testing methods

resection samples

NCCN guidelines