2023 World Conference on Lung Cancer (Posters)-EP13.01. Single-Cell RNA Sequencing Reveals Immune Microenvironment of Small Cell Lung Cancer-associated Malignant Pleural Effusion

EP13.01. Single-Cell RNA Sequencing Reveals Immune Microenvironment of Small Cell Lung Cancer-associated Malignant Pleural Effusion - PDF(Slides)

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Researchers used single-cell RNA sequencing to explore the immune microenvironment of small cell lung cancer (SCLC) associated malignant pleural effusion (MPE). The study aimed to provide insights into the features of disseminated tumor cells from SCLC and their immune microenvironment.<br /><br />The researchers obtained pleural effusion samples from a patient with SCLC-originated MPE. Using 10x genomics single-cell transcriptome sequencing, they constructed a gene library for analysis. A bioinformatics analysis was then performed to reveal the characteristics of the tumor immune microenvironment.<br /><br />A total of 8,008 high-quality cells were obtained from the analysis. These cells were further classified into 10 clusters, which were identified as B cells (clusters 3, 5, and 7), T cells (clusters 1, 2, and 6), myeloid cells (cluster 4), NK cells (cluster 8), and cancer cells (clusters 9 and 10). Within the B cell clusters, subgroups of translational B cells, memory B cells, naive B cells, and plasma B cells were identified. Within the T cell clusters, subgroups of naive CD8 T cells, CD8 effector_GZMK T cells, CD8 effector_GNLY T cells, naive CD4 T cells, CD4 effector T cells, CD4 memory T cells, and Treg cells were identified.<br /><br />The researchers used monocle cell trajectory analysis to explore the differentiation of T and B cells. They found that several SCLC-related genes were expressed at different levels during this process. Cell communication analysis revealed a complex interaction network between T cells, B cells, and tumor cells. The interactions between these cell types mainly occurred through ligands and receptors such as MHC II, IL-16, GALECTIN, and APP signaling pathway.<br /><br />In conclusion, this study identified the composition of immune cells in MPE of patients with SCLC. It also revealed the communication pathways between immune cells and tumor cells, which may have implications for the efficacy of immunotherapy. Further research is needed to understand the relationship between these pathways and the effectiveness of immunotherapy.

Asset Subtitle

Shuyan Wang

Meta Tag

Speaker Shuyan Wang

Topic SCLC & Neuroendocrine Tumors: Biomarkers & Radiomics

Keywords

single-cell RNA sequencing

immune microenvironment

small cell lung cancer

malignant pleural effusion

disseminated tumor cells

10x genomics single-cell transcriptome sequencing

bioinformatics analysis

B cells

T cells

myeloid cells