2023 World Conference on Lung Cancer (Posters)-P1.07. Collective Lung Cancer Migration Using a 3D Model

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This research study presented at the WCLC 2023 conference focuses on the migration patterns of lung cancer cells using a 3D model. The study found that pulmonary adenocarcinomas and squamous cell carcinomas preferentially migrate in bulks of up to 200 cells. This type of migration contributes to early recurrence and metastasis. The researchers developed a 3D bioprinter to create a lung-specific matrix in cell culture wells, and then layered cultured cell lines from adenocarcinoma and small cell carcinoma on top of the matrix to study migration. <br /><br />The results showed that the adenocarcinoma formed cell clusters and started invading the matrix after one week. The tumor cells also started to digest the matrix. Immunohistochemistry was performed to evaluate the expression of migration and epithelial-mesenchymal transition (EMT) associated molecules. The study found that Twist, Mad, Brk, and Rack1 were expressed in both leader and follower cells of the adenocarcinoma, while Snail and Slug were not expressed, indicating a hybrid-EMT pattern. The small cell carcinoma, on the other hand, migrated in small groups with no leader or follower differentiation and showed a more classical EMT expression pattern. <br /><br />The researchers concluded that adenocarcinoma cells migrate in bulks and express hybrid-EMT associated molecules, while small cell carcinomas show a different migration behavior and a classical EMT expression pattern. They also highlighted the usefulness of 3D models in studying migration of lung cancer cells, as they closely resemble findings within the tissues. <br /><br />The keywords associated with this study are pulmonary adenocarcinoma, bulk/collective migration, and migration-associated molecules. This research falls under the track of tumor biology and translational biology.

Asset Subtitle

Helmut Popper

Meta Tag

Speaker Helmut Popper

Topic Tumor Biology: Translational Biology - Disease Modeling

Keywords

lung cancer cells

3D model

migration patterns

pulmonary adenocarcinomas

squamous cell carcinomas

early recurrence

metastasis

3D bioprinter

hybrid-EMT pattern

classical EMT expression pattern