2023 World Conference on Lung Cancer (Posters)-P1.07. Collective Lung Cancer Migration Using a 3D Model

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This study aimed to establish a 3D bio-printed model of lung cancer to investigate migration and invasion associated molecules and simulate in vivo conditions. The 3D model was created using a bio-printer from TissueLabs and consisted of an alveolar-like structure with channels. Two cell lines, A549 and NCI-H82, were seeded onto the matrix and incubated for several days.<br /><br />Immunohistochemical staining was performed on the 3D bio-printed model to examine the expression of various migration-related markers. In the A549 cell line, positive staining for keratin indicated epithelial cells, while markers such as Mad, Brk, and Vimentin were associated with invasion and migration. Vimentin staining showed both positive leader cells and negative follower cells within the adenocarcinoma bulk. Rack1 stained all cells but with different intensities. Unexpectedly, the marker TKs5 was negative. The markers Slug and Snail, associated with epithelial to mesenchymal transition, as well as tumor cell proliferation marker Twist, were all negative.<br /><br />In the NCI-H82 cell line, positive staining for keratin indicated epithelial origin, while Vimentin staining was completely negative, which was not surprising for small cell lung cancer cells.<br /><br />The established 3D bio-printed model provides a valuable tool for studying tumor migration and invasion in lung cancer research. This model can help reduce the need for animal experiments in basic research and contribute to the understanding of cancer biology and the development of anti-tumor therapeutics. The study was financially supported by Astra Zeneca, Hofman La Roche, and "Value Privatstiftung" and the authors acknowledge the technical assistance of the BSL-3 laboratory team at the Medical University of Graz.

Asset Subtitle

Helmut Popper

Meta Tag

Speaker Helmut Popper

Topic Tumor Biology: Translational Biology - Disease Modeling

Keywords

3D bio-printed model

lung cancer

migration

invasion

molecules

in vivo conditions

TissueLabs

alveolar-like structure

A549 cell line

NCI-H82 cell line