2023 World Conference on Lung Cancer (Posters)-P1.24. Discovery of Novel Biomarkers of Small Cell Lung Cancer Using Highly Sensitive Quantitative Proteomic Analysis

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Researchers from Kyung Hee University College of Medicine and Daegu Gyeongbuk Institute of Science and Technology (DGIST) have discovered novel biomarkers for small cell lung cancer (SCLC) using highly sensitive quantitative proteomic analysis. The study involved analyzing the proteomes of bronchoalveolar lavage fluid (BALF) samples collected from SCLC patients. The researchers found that several proteins were elevated in tumor-bearing BALF, with CNDP2 and RNPEP identified as potential indicators for specific subtypes of SCLC. The study also showed a positive correlation between CNDP2 and chemo-drug responses, which could help inform treatment decisions for SCLC patients.<br /><br />The researchers performed tandem mass scanning and used MaxQuant software to identify a total of 460 proteins in the BALF samples. Principal component analysis revealed distinct clusters based on patients rather than tumor-bearing status. The study also highlighted the challenges of analyzing BALF proteomes due to the potential contamination of blood proteins.<br /><br />To address these challenges, the researchers used immunoaffinity-based depletion of highly abundant proteins followed by peptide fractionation, which significantly improved the identification of lung cancer biomarkers from BALF. The study demonstrated the heterogeneity of BALF proteomes among SCLC patients using sensitive silver staining and further investigated this difference using tandem mass tags (TMT)-based quantitative proteomic analysis.<br /><br />Gene ontology analysis revealed that the identified proteins in the BALF samples were associated with functions related to endopeptidase inhibitors, actin-binding, and negative regulation of endopeptidase activity, indicating their roles in inflammation regulation and immune response.<br /><br />The study validated the elevated proteins discovered in tumor-bearing BALF using immunohistochemistry of SCLC tissues and demonstrated the potential of CNDP2 and RNPEP as subtype markers for specific SCLC subtypes, namely ASCL1 and NEUROD1. Furthermore, the study showed that CNDP2 was positively correlated with responses to certain chemotherapy drugs.<br /><br />Overall, this study contributes to the understanding of SCLC biomarkers and may have implications for the diagnosis, prognosis, and treatment of SCLC patients.

Asset Subtitle

Seung Hyeun Lee

Meta Tag

Speaker Seung Hyeun Lee

Topic Pathology & Biomarkers: Prognostic Biomarkers

Keywords

small cell lung cancer

biomarkers

quantitative proteomic analysis

bronchoalveolar lavage fluid

CNDP2

RNPEP

chemo-drug responses

tandem mass scanning

MaxQuant software

immunoaffinity-based depletion