2023 World Conference on Lung Cancer (Posters)-P2.18. Successful Generation of Tumor-Infiltrating Lymphocytes (TIL) for Adoptive Cell Therapy from Mesothelioma

P2.18. Successful Generation of Tumor-Infiltrating Lymphocytes (TIL) for Adoptive Cell Therapy from Mesothelioma - PDF(Slides)

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Researchers have successfully generated tumor-infiltrating lymphocytes (TIL) for adoptive cell therapy from mesothelioma, a type of cancer with a poor prognosis. TIL cell therapy involves expanding T cells from tumor tissue, creating a diverse T-cell product capable of recognizing tumor-specific antigens. The study used a 22-day manufacturing process to generate TIL from mesothelioma tumors and characterized their phenotypic and functional characteristics.<br /><br />The study included six patients with mesothelioma, and five of the final TIL products (83%) met the acceptance criteria for TIL product attributes. The median yield of TIL on day 11 was 41 million cells, and on day 22 was 53 billion viable cells. The TIL generated from mesothelioma tumors showed acceptable attributes and were comparable in function and phenotype to TIL generated from other tumor types.<br /><br />The TIL predominantly expressed TCR α/β and a mix of CD4 and CD8 T-cells. B cells and monocyte populations were not observed in the final TIL product, while the proportion of NK cells was very low. The TIL populations expressed higher levels of activation/exhaustion markers compared to differentiation markers, and predominantly had an effector memory phenotype.<br /><br />The TIL released interferon-gamma (IFNγ) in response to stimulation, with median levels of 4454 pg/mL. These results are similar to historical results in melanoma, indicating that the TIL generated from mesothelioma tumors have functional activity.<br /><br />Overall, the study suggests that viable TIL can be successfully expanded from mesothelioma tumor tissue using the described manufacturing process. This opens up the potential for clinical investigation of TIL cell therapy in patients with mesothelioma, providing a potential treatment option for this difficult-to-treat cancer.

Asset Subtitle

Dean Fennell

Meta Tag

Speaker Dean Fennell

Topic Mesothelioma, Thymoma & Other Thoracic Tumors: Clinical

Keywords

tumor-infiltrating lymphocytes

adoptive cell therapy

mesothelioma

T-cell product

manufacturing process

phenotypic characteristics

functional characteristics

effector memory phenotype

interferon-gamma

difficult-to-treat cancer